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KMID : 0525720200250040175
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Journal of Chitin and Chitosan
2020 Volume.25 No. 4 p.175 ~ p.183
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Glycation Inhibitory Effect and Renal Protective Ability of Hizikia Fusiformis Extracts Against Advanced Glycation End-products (AGEs)
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Kim Min-Gyeong
Cho Chi-Heung
Youm Ga-Hee
Park Yong-Kon
Lee Sang-Hoon
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Abstract
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In this study, we evaluated the anti-glycation effects and renal protective properties of Sargassum fusiforme extract against advanced glycation end-products (AGEs)-induced oxidative stress. Among the extracts of S. fusiforme with different concentrations of ethanol (0, 10, 30, 70, and 95%(v/v)), 70% aqueous ethanol extract of S. fusiforme (70% EtOH SFE) showed the highest total phenolics, condensed tannin, and total flavonoid contents (0.797±0.036 mg GAE/g DW, 0.298±0.009 mg CE/g DW, 0.381±0.060 mg CE/g DW, respectively). All tested extracts of S. fusiforme showed concentration-dependent anti-glycation activity, in particular, 70% EtOH SFE exhibited the most prominent anti-glycation effects in all in vitro experiments. 70% EtOH SFE inhibited 7.13% AGEs-BSA and collagen cross-link at a concentration of 100 μg/mL , which was significantly (p < 0.05) higher than aminoguanidine (36.9%.), the well-known cross-link inhibitor. 70% EtOH SFE (100 μg/mL) showed AGEs-BSA and collagen cross-link breaking activity to the 17.9%. Additionally, 70% EtOH SFE inhibited formation of AGEs in a concentration-dependent manner up to 66.8%. Furthermore, treatment with 70% EtOH SFE not only increased the viability of methylglyoxal (MGO)-induced mesangial cells to 64.9%, but also dramatically reduced the intracellular oxidative stress level to 94.9%. The treatment of 70% EtOH SFE was decreased MGO-induced apoptotic cell death by 21.9% while aminoguanidine by 33.4%. Consequently, our results suggest that 70% EtOH SFE may be a good source of natural therapeutic agent for management of diabetic complications via anti-glycation effect and renal protective ability against MGO-induced oxidative stress.
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KEYWORD
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Sargassum fusiforme, Marine algae, Advanced glycation end-products, Renoprotective effect, Diabetic complications
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